CO-EXISTENCE OF CIPROFLOXACIN RESISTANCE IN METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS FROM CLINICAL SAMPLES
Background: Ciprofloxacin is a broad-spectrum, fluorinated quinolone antibiotic. It has been successfully prescribed against a variety of bacteria. Though its use was limited to a last resort therapy against complicated infections, however widespread usage has led to the emergence of ciprofloxacin resistance. The current study was planned to evaluate the existence of ciprofloxacin resistance in methicillin resistant and sensitive Staphylococcus aureus from clinical samples. Methods: The clinical S. aureus isolates from wound, blood, urine and nose, were obtained from various labs of Hyderabad over nine months. The methicillin resistant strains were identified by Kirbey baur disc diffusion test using oxacillin (1 µg) discs. The response of Methicillin Resistant S. aureus and Methicillin Sensitive S. aureus strains against 2nd generation flouroquinolone, i.e., ciprofloxacin was demonstrated. Results: A total of 150 S. aureus isolates from various clinical specimens were included in this study. About 14.6 % (n=22) showed resistance against ciprofloxacin while 30% (n=48) were identified as MRSA. About 25% of the Methicillin Resistant S. aureus (MRSA) isolates displayed the co-resistance against ciprofloxacin. Among various specimens the maximum co-resistance was seen in case of blood isolates (37.5%) followed by urine isolates (33.3%). Comparatively, ciprofloxacin resistance was found to be lower in Methicillin Sensitive S. aureus (MSSA) strains (9.1%). Odds Ratio [OR] was calculated to be 0.30 [95% CI=0.12–0.77]. Statistically significant differences (p˂0.05) for ciprofloxacin resistance were seen between MRSA and MSSA. Conclusion: The results suggest a statistically significant increase of ciprofloxacin resistance in Methicillin Resistant S. aureus as compared to Methicillin Sensitive S. aureus in clinical samples.
Pak J Pysiol 2018;14(2)24–7
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