MODULATION OF VISFATIN LEVELS BY THIAZOLIDINEDIONES IN INSULIN RESISTANT SPRAGUE DAWLEY RATS
Background: Insulin resistance is manifested by decreased effect of fixed quantity of insulin on glucose metabolism leading to type 2 diabetes mellitus. Visceral obesity has a positive correlation with insulin resistance but its mechanism is not fully defined. Insulin resistance may be the consequence of adipocytokines including visfatin and resistin. This study was designed to see the effect of thiazolidinediones on visfatin levels in insulin resistant rats. Methods: Ninety Sprague Dawley rats were divided into 3 groups of 30 each. Group I served as control. Rats in Group II and III were made insulin resistant diabetics with high sucrose diet. Group III was treated with thiazolidinediones after development of diabetes. Plasma glucose, serum triglycerides, HDL, TG:HDL ratio and serum visfatin levels were analysed. Results: Body weight and plasma glucose, visfatin levels and TG:HDL ratio were significantly increased (p<0.05) in group II and group III at the end of 4th week. Treatment of group III with thiazolidinediones led to improvement in insulin resistance with increase in serum visfatin levels (p<0.05). Conclusion: Thiazolidinediones augment sensitivity of insulin to restore normoglycaemia by increasing serum visfatin level.
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2. Hameed W, Yousaf I, Latif R, Aslam M. Effect of visfatin on testicular steroidogenesis in diabetic rats and isolated Leydig cell culture. J Ayub Med Coll Abbottabad 2012;24(3-4):62â€“4.
3. Chen MP, Chung FM, Chang DM, Tsai JC, Huang HF, Shin SJ. Elevated plasma level of visfatin/pre-B cell colony-enhancing factor in patients with Type 2 diabetes mellitus. J Clin Endocrinol Metab 2006;91:295â€“9.
4. Samaras K, Campbell LV. Increasing incidence of type 2 diabetes in the third millennium: is abdominal fat the central issue? Diabetes Care 2000;23:441â€“2.
5. Olefsky JM. Treatment of insulin resistance with peroxisome proliferator-activated receptor gamma agonists. J Clin Invest 2000;106:467â€“72.
6. Yassin MM, Mwafy SN. Protective potential of glimepiride and Nerium oleander extract on lipid profile, body growth rate, and renal function in streptozotocin-induced diabetic rats. Turk J Biol 2007;31:95â€“102.
7. Gutman RA, Basilico MZ, Bernal A, Chicco A, Lombardo YB. Long-term hypertriglyceridemia and glucose intolerance in rats fed chronically an isocaloric sucrose-rich diet. Metabolism 1987;36:1013â€“20.
8. Hwang IS, Ho H, Hoffman BB, Reaven GM. Fructose-induced insulin resistance and hypertension in rats. Hypertension 1987;10:512â€“6.
9. Hulman S, Falkner B. The effect of excess dietary sucrose on growth, blood pressure, and metabolism in developing Sprague Dawley rats. Pediatr Res 1994;36(1 Pt 1):95â€“101.
10. Dominik G, Haider I, Friedrich MI, Georg SI, Michaela A, Sabina M. Free fatty acids normalize a rosiglitazone-induced visfatin release. Am J Physiol Endocrinol Metab 2006;291:E885â€“90.
11. Choi KC, Ryu OH, Lee KW. Effect of PPAR-Î± and -Î³ agonist on the expression of visfatin, adiponectin, and TNF-Î± in visceral fat of OLETF rats. Biochem Biophys Res Commun 2005;336:747â€“53.
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