EFFECT OF WHITE SUGAR, BROWN SUGAR AND JAGGERY POWDER SYRUP ON BIOCHEMICAL AND HISTOPATHOLOGICAL MARKERS OF SPRAGUE-DAWLEY RATS

Authors

  • Hifza Noor Lodhi Central Park Medical College, Lahore, Pakistan
  • Sana Rasheed Chaudry Postgraduate Medical Institute, Lahore, Pakistan
  • Shagufta Nasreen Postgraduate Medical Institute, Lahore, Pakistan
  • Amer Hassan Siddiqui Postgraduate Medical Institute, Lahore, Pakistan
  • Rana Muhammad Yousaf Lahore General Hospital, Lahore, Pakistan
  • Sadia Chiragh Al-Aleem Medical College, Lahore, Pakistan
  • Muniza Saeed Postgraduate Medical Institute, Lahore, Pakistan

Keywords:

Sugar, Srprague_Dawley rats, histopathological markers, Biochemical markers

Abstract

Hypertension accounts for 10% of the total annual health budget in developed countries.1 By 2025, the number of people living with hypertension is expected to reach 1.56 billion people.2Dietary factors that increase blood pressure (BP) are of interest to public health authorities.3,4Twenty adult male Sprague-Dawley rats were taken. All groups received standard chow ad libitum. Controls (A), received plain drinking water, and the Sucrose treatment rats received 10% sucrose solution prepared daily for 12 weeks. Non-invasive blood pressure was recorded  using computer based data recording system power lab model: M-ML 856, Australia with rat tail cuff attachment.Blood samples were collected. Serum aliquots were frozen at ?80°C for: ALT, AST, TG, total cholesterol (TC), and high-density lipoprotein (HDL) were determined using commercial enzymatic methods (CELM diagnosis, São Paulo, Brazil). The concentration of VLDL and low-density lipoprotein was measured.

ANOVA and Post-Hoc Tukey tests were applied to determine statistical difference among the study groups.

The results showed variable effects on biochemical and histopathological findings in rat liver, kidney and pancreas. The pancreas and kidneys of the rats showed adverse inflammatory histopathological changes maximally in rats taking jaggery syrup (Shakkar) flowed by white sugar and minimal in Shakkar. The adverse effects on liver cells were maximally seen on rats given brown sugar.

This study enabled us to better understand the biochemical and histopathological effects of various types of sugars available in Pakistani market in order to create awareness regarding their effects on health.

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Author Biographies

Sana Rasheed Chaudry, Postgraduate Medical Institute, Lahore, Pakistan

assistant prof Physiology

Shagufta Nasreen, Postgraduate Medical Institute, Lahore, Pakistan

assistant prof Anatomy

Amer Hassan Siddiqui, Postgraduate Medical Institute, Lahore, Pakistan

assistant pro Pharmacology

Rana Muhammad Yousaf, Lahore General Hospital, Lahore, Pakistan

Incahrge central research Lab

Sadia Chiragh, Al-Aleem Medical College, Lahore, Pakistan

prof. of pharmacology

Muniza Saeed, Postgraduate Medical Institute, Lahore, Pakistan

prof of physiology

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Published

31-03-2024

How to Cite

1.
Lodhi HN, Chaudry SR, Nasreen S, Siddiqui AH, Yousaf RM, Chiragh S, et al. EFFECT OF WHITE SUGAR, BROWN SUGAR AND JAGGERY POWDER SYRUP ON BIOCHEMICAL AND HISTOPATHOLOGICAL MARKERS OF SPRAGUE-DAWLEY RATS. Pak J Phsyiol [Internet]. 2024 Mar. 31 [cited 2024 Jun. 17];20(1):3-7. Available from: https://www.pjp.pps.org.pk/index.php/PJP/article/view/1527