COMPARISON OF SALIVARY ANTIOXIDANT LEVEL IN SMOKERS AND NON-SMOKERS
DOI:
https://doi.org/10.69656/pjp.v21i1.1803Keywords:
Antioxidant, Oxidative Stress, Salivary alpha-amylase, Uric acidAbstract
Background: Saliva is the first biological medium encountered during inhalation of cigarette smoke. Uric acid in saliva possesses free-radical-scavenging properties which are produced by cigarette smoke and contributes approximately 70–85% of the total antioxidative capacity of saliva. Objective of this study was to determine and compare the salivary uric acid (SUA) level in cigarette smokers and non-smokers amongst the patients visiting Sardar Begam Dental Teaching Hospital, Peshawar. Methods: It was a comparative study done on 118 individuals, (59 in each group of cigarette smokers and non-smokers). Non-probability convenience sampling was used. The data was collected using structured proforma and analysed using SPSS-25. For the association of quantitative variables with smoking independent sample t-test was used. Chi-square test was used for qualitative variables, and p?0.05 was considered statistically significant. Results: Mean salivary uric acid level in cigarettes smokers was 2.7±1.02 mg/dL as compared to 4.7±0.91 mg/dL in non-smokers. Conclusion: Salivary uric acid level was found significantly lower in cigarette smokers compared with non-smokers.
Pak J Physiol 2025;21(1):100-2, DOI: https://doi.org/10.69656/pjp.v21i1.1803
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Fatima G, Uppin RB, Kasagani S, Tapshetty R, Rao A. Comparison of salivary uric acid level among healthy individuals without periodontitis with that of smokers and non-smokers with periodontitis. J Adv Oral Res 2016;7:24–8.
Fábián TK, Hermann P, Beck A, Fejérdy P, Fábián G. Salivary defense proteins: Their network and role in innate and acquired oral immunity. Int J Mol Sci 2012;13:4295–320.
Xu R, Cui B, Duan X, Zhang P, Zhou X, Yuan Q. Saliva: potential diagnostic value and transmission of 2019-nCoV. Int J Oral Sci 2020;12:1–6.
Dawes C, Pedersen AL, Villa A, Ekström J, Proctor GB, Vissink A, et al. The functions of human saliva: A review sponsored by the World Workshop on Oral Medicine VI. Arch Oral Biol 2015;60(6):863–74.
Laine MA, Tolvanen M, Pienihäkkinen K, Söderling E, Niinikoski H, Simell O, et al. The effect of dietary intervention on paraffin-stimulated saliva and dental health of children participating in a randomized controlled trial. Arch Oral Biol 2014;59:217–25.
Castagnola M, Scarano E, Passali GC, Messana I, Cabras T, Iavarone F, et al. Salivary biomarkers and proteomics: Future diagnostic and clinical utilities. Acta Otorhinolaryngol Ital 2017;37:94–101.
Pullishery F. Salivary thiocyanate, uric acid and pH as biomarkers of periodontal disease in tobacco users and non-users —an in-vitro study. J Clin Diagn Res 2015;9:ZC47–50.
Diab-Ladki R, Pellat B, Chahine R. Decrease in the total antioxidant activity of saliva in patients with periodontal diseases. Clin Oral Investig 2013;7:103–7.
Novakovic N, Todorovic T, Rakic M, Milinkovic I, Dozic I, Jankovic S, et al. Salivary antioxidants as periodontal biomarkers in evaluation of tissue status and treatment outcome. J Periodontal Res 2014;49:129–36.
González-Hernández JM, Franco L, Colomer-Poveda D, Martinez-Subiela S, Cugat R, Cerón JJ, et al. Influence of sampling conditions, salivary flow, and total protein content in uric acid measurements in saliva. Antioxidants 2019;8:386.
Munther S. The effects of cigarette smoking and exercise on total salivary antioxidant activity. Saudi Dent J 2019;31:31–8.
Schwartz M, Neiers F, Feron G, Canon F. The relationship between salivary redox, diet, and food flavor perception. Front Nutr 2021;7:612735.
Ahmadi-Motamayel F, Amjad SV, Goodarzi MT, Poorolajal J. Evaluation of salivary uric acid and pH in human immunodeficiency virus infected patients: A historical cohort study. Infect Disord Drug Targets 2017;18:35–40.
Jenifer HD, Bhola S, Kalburgi V, Warad S, Kokatnur VM. The influence of cigarette smoking on blood and salivary super oxide dismutase enzyme levels among smokers and nonsmokers —A cross sectional study. J Tradit Complement Med 2015;5:100–5.
Khozeimeh F, Torabinia N, Shahnaseri S, Shafaee H, Mousavi S. Determination of salivary urea and uric acid of patients with halitosis. Dent Res J (Isfahan) 2017;14:241–5.
Zalewska A, Klimiuk A, Zi?ba S, Wnorowska O, Rusak M, Waszkiewicz N, et al. Salivary gland dysfunction and salivary redox imbalance in patients with Alzheimer’s disease. Sci Rep 2021;11:23904.
Indrastiti RK, Rahmayanti F, Soegyanto AI. Analysis of the total antioxidant capacity of saliva in smokers. Pesqui Bras Odontopediatria Clin Integr 2019;19:48–97.
Bilancio G, Cavallo P, Lombardi C, Guarino E, Cozza V, Giordano F, et al. Saliva for assessing creatinine, uric acid, and potassium in nephropathic patients. BMC Nephrol 2019;20:242.
Iwai K, Minamisawa T, Suga K, Yajima Y, Shiba K. Isolation of human salivary extracellular vesicles by iodixanol density gradient ultracentrifugation and their characterizations. J Extracell Vesicles 2016;5:308–29.
Bakhtiari S, Toosi P, Samadi S, Bakhshi M. Assessment of uric acid level in the saliva of patients with oral lichen planus. Med Princ Pract 2017;26:57–60.
Hanna BE, Hamed JM, Touhala LM. Serum uric acid in smokers. Oman Med J 2008;23:269–74.
Greabu M, Totan A, Battino M, Mohora M, Didilescu A, Totan C, et al. Cigarette smoke effect on total salivary antioxidant capacity, salivary glutathione peroxidase and gamma-glutamyltransferase activity. BioFactors 2008;33:129–36.
Zappacosta B, Persichilli S, De Sole P, Mordente A, Giardina B. Effect of smoking one cigarette on antioxidant metabolites in the saliva of healthy smokers. Arch Oral Biol 1999;44:485–8.
Miricescu D, Greabu M, Totan A, Didilescu A, Radulescu R. The antioxidant potential of saliva: Clinical significance in oral disease. Ther Pharmacol Clin Toxicol 2011;15;139.
Abdolahinia A, Sadr M, Hessami Z. View of correlation between the age of smoking initiation and maintaining continuous abstinence for 5 years after quitting. Acta Med Iran 2012;50:755–9.
Abdolsamadi HR, Goodarzi MT, Mortazavi H, Robati M, Ahmadi-Motemayel F. Comparison of salivary antioxidants in healthy smoking and non-smoking men. Chang Gung Med J 2011;34:607–18.
Dube SR, Liu J, Fan AZ, Meltzer MI, Thompson WW. Assessment of age-related differences in smoking status and health-related quality of life (HRQoL): Findings from the 2016 Behavioral Risk Factor Surveillance System. J Community Psychol 2019;47:93–103.
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